Sample quantity: The kit to be used depends on the size of the sample being analysed.Humic content: If the sample has humic content such as compost, sediment and manure, a kit/method that removes humic substances should be used, as they can inhibit downstream applications like PCR.Intended use: The quality and purity of the DNA provided by the kit should be suitable for the intended downstream application, which could be sequencing, fingerprinting, PCR, quantitative PCR (qPCR), Southern blotting, random amplification of polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP) applications, restriction endonuclease digestion, or the preparation of shotgun libraries.Preparation method: Sample preparations can be: fresh or previously frozen cell pellets, paraffin-embedded or formalin-fixed tissue sections, frozen tissue sections, ethanol-fixed cells, Oragene®-preserved samples, and samples from forensic sources which might contain very limited material.Sample origin: Different kits are used to extract material from specific sources, including human tissues, blood, hair, rodent tissues, leaf tissue, bacteria, yeast, fungi, insect, stool, body fluids, spores, soil, clinical samples (e.g., biopsy samples, fine needle aspirates), forensic samples (e.g., dried blood spots, buccal swabs), and fingerprints.Factors to be considered for selecting a kit include:
Basic steps involved in all DNA extraction methods.Ĭhoosing the correct DNA extraction kit can save crucial time on optimization and execution of the experiment.
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